| Title |
Characteristics of Indium-Tin-Oxide Electrode for Continuous-flow PCR Chip |
| Authors |
정승룡(Joung, Seung-Ryong) ; 김준혁(Kim, Jun-Hyeok) ; 이인제(Yi, In-Je) ; 강치중(Kang, C.J.) ; 김용상(Kim, Yong-Sang) |
| Keywords |
continusous-flow PCR chip ; polydimethylsiloxane ; indium-tin-oxide electrode |
| Abstract |
We propose glass and PDMS (polydimethylsiloxane) chips for DNA amplification with continuous-flow PCR (polymerase chain reaction). The PDMS microchannel was fabricated using a negative molding method for sample injection. Three heaters and sensors of ITO (indium-tin-oxide) thin films were fabricated on glass chip. ITO heaters and sensors were calibrated accurately for the temperature control of the liquid flow. ITO heater generated stable heat versus applied power. ITO sensor resistance was changed linearly versus temperature increase as a RTD (resistance temperature detector) sensor. As a result, we enable precision temperature control of continuous-flow PCR chip. Using the continuous-flow PCR chip DNA plasmid pKS-GFP 720 bp was successfully amplified. |