| Title |
A Disposable Grating-Integrated Multi-channel SPR Sensor Chip for Detection of Biomolecule |
| Authors |
진영현(Jin, Young-Hyun) ; 조영호(Cho, Young-Ho) |
| Keywords |
SPR sensor chip ; Grating ; biomolecule detection ; Detection of biomolecule concentration |
| Abstract |
This paper presents a grating~integrated SPR (Surface Plasmon Resonance) sensor chip for simple and inexpensive biomolecule detection. The grating-integrated SPR sensor chip has two sensing channels having a nano grating for SPR coupling. An external mirror is used for multi channel SPR sensing. The present sensor chip replaces bulky and expensive optical components, such as fiber-optic switches or special shaped prisms, resulting in a simple and inexpensive wavelength modulated multi-channel SPR sensing system. We fabricate a SPR sensor chip integrated with 835 nm-pitch gratings by a micromolding technique to reduce the fabrication cost. In the experimental characterization, the refractive index sensitivity of each sensing channel is measured as 321.8pm8.1nm/RI and 514.3pm8.lnm/RI, respectively. 0.5uM of the target biomolecule (streptavidin) was detected by a 1.13pm0.16nm shift of the SPR dip in the 10%-biotinylated sample channel, while the SPR dip in the reference channel for environmental perturbation monitoring remained at the same position. From the experimental results, multi-channel biomolecule detection capability of the present grating-integrated SPR sensor chip has been verified. On the basis of the preliminary experiments, we successfully measured the binding reaction rate for the 2 nM~200 nM monoclonal-antibiotin, thus verifying biomolecule concentration detectability of the present SPR sensor chip. The binding reaction rates measured from the present SPR sensor chip agredd well with those from a commercialized SPR sensor. |